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Project title: Tetrapyrrole nanostructures towards fluorescent molecular markers for biomedicine Acronim: Bio-Mark Program: MNT-ERA.NET Project duration: 2010-2012 Total costs: 575000 € Romanian part costs: 295000 €
Project coordinator Prof. Dr. Luis Filipe Vieira Ferreira Instituto Superior Técnico – Technical University of Lisbon – Centro de Química-Física Molecular e Instituto de Nanociência e Nanotecnologia Lisbon, Portugal E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Partners 1. Escola Superior de Tecnologia e Gestão do Instituto Politécnico de Portalegre, Portugal 2. ,,Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania 3. Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, Bucharest, Romania 4. “Victor Babes” National Institute of Pathology
Romanian coordinator ,,Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania Project Manager: Conf. Dr. Rica Boscencu E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it Links
Project summary During the last decades the scientific community made sustained efforts to obtain new diagnosis medical devices for the investigation of the metabolism and/or cell/tissue functions so as to enable the differentiation pf pathologic disturbances in early stages of the disease. Nanostructured tetrapyrrole structures are among the compounds investigated in view of thei use as fluorescent markers. This new field of use for porphyrins was partially steamed by their application as non-conventional medical devices in photodynamic therapy (PDT), as they exhibit convenient absorption in the phototherapeutic window (~670 to 1100nm). The compounds designed for diagnosis via photosensitization have to display great quantum yields, great Sokes shifts, reduced photolability while presenting optimum characteristics to be used in studies on cells and tissues (well balanced amphyphilic characteristics, without intrinsic toxicity, greater excitation wavelengths such as to avoid spectral interferences due to the biologic environmental autofluorescence). The porphyrinic structures exhibit long lifetime values, which grants them an extra-advantage for fluorescence imagistics. The main goal of the project is to obtain tetrapyrrole nanostructures to be used as NIR fluorescent probes for microscopy studies on normal and pathologic cells, i.e. primary cells (immune cells, keratinocytes) and standard cell lines. The overall objectives will focus on the synthesis of newly tetrapyrrolic structures, on their complex characterisation (including their ability to penetrate the cell membrane, their localisation inside the cell and on the overall effects of the compounds on the cell properties and/or functions). The studies will emphasize the interaction of the compounds with the human keratinocytes, known to be more resistant to xenobiotic intake, compared to standard cells (normal and modified cell lines).
Published and communicated results (2010, 2011)
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Informatiile oficiale pot fi gasite la avizierele si secretariatele U.M.F. "Carol Davila"